Diabetic retinopathy treatment protocol. Modeling long-term diabetes and related complications in rats


  • Men and women with newly diagnosed active CNV secondary to AMD, diagnosed by a retinal specialist with all the following characteristics and ophthalmic inclusion criteria applied to the study eye, as assessed by a central reader.
  • Modeling long-term diabetes and related complications in rats
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Modeling long-term diabetes and related complications in rats J Pharmacol Toxicol Methods. Mar-Apr ; Epub Nov Gyömrői út Electronic address: erika. DOI: The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the pathological changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia.

Methods: Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control Diabetic groups. Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests.

diabetic retinopathy treatment protocol

Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electron microscopy.

Discussion: We established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period.

Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical diabetic retinopathy treatment protocol. Our results provide a useful basis for designing studies for testing preventative treatments as well as other translational medical research in this field.

diabetic retinopathy treatment protocol

Keywords: Anterior segment neovascularization; Chronic model; Cold allodynia; Diabetic nephropathy; Diabetic neuropathy; Diabetic retinopathy; Drug development; Insulin implant; Preclinical model; Streptozotocin. Copyright © Elsevier Inc.

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diabetic retinopathy treatment protocol